Image Tools
Image Tools
Image tools provides a number of options to enable you to optimise the view of the current image:
- 2D Displays the image in a single two dimensional (2D) view. This renders a single Z slice at a single time point.
- 2D MIP (Maximum Intensity Projection) Displays the image as a top down view through the entire Z stack by rendering the brightest voxels. This is for a single time point.
- 2D Split Renders up to three separate channels individually and a combined multi-channel view at the same time in four quadrants.
- 2D Split Channel MIP Renders the image as a top down view through the entire Z stack by rendering the brightest voxels for up to three channels individually and a combined multi-channel view at the same time in four quadrants..
- 3D MIP This renders an interactive 3D volume view that appears translucent as the brightest objects are rendered.
- 3D Blend Sets the display to a three dimensional blend mode. This combines all image intensities along the viewing direction to produce a solid effect to the image. The opacity of the voxels varies according to their intensity- the lowest intensity is transparent.
Reset 3D View Resets the 3D view to the original state after the image has been rotated or moved.
Multiple image display icon( in green)
- displays all the images of a data set at the same time.
- The display can be done in real-time.
- The display is very useful for visualising progress on a Multi-Well experiment.
Image Zoom Controls
The Image Zoom controls are located at the bottom of the main image viewing window.
- Channel visibility Control the visibility of the currently selected channel. Right click the box (in this example the channel is displayed in yellow) to bring up the Look Up Table (LUT) for the channel.
- Multi. Select to display a single channel, or select Multi to display multiple channels (where available).
- Scale Bar Displays the scale bar on top of the current on-screen image in any 2D mode.
- Zoom. Set the zoom level for the current on-screen image. At 100% zoom, 1 image voxel is equivalent to 1 on-screen pixel.
- Pos. Hover the mouse pointer over a point of interest and the x and y co-ordinates are displayed (in this example 401, 132). Adjacent to this the pixel intensity is displayed in brackets (in this example, 104). This value is always the true pixel intensity as captured by the camera. It is unaffected by mapping. It may not be available in all views - for example, 3D view modes. Note - the image axes origin in Fusion are the bottom left corner. This is the same for Imaris. Other image programs may have their origin as the top left corner.
- x & y Displayed the dimensions of the current on-screen image. In this example 512 x 512.
For further information refer to the section Image Visualisation.